Subcellular-level resolution MALDI-MS imaging of maize leaf metabolites by MALDI-linear ion trap-Orbitrap mass spectrometer


Publication Type:

Journal Article


Analytical and Bioanalytical Chemistry (2015)

Other Number:

PMID 25618761


<p>A significant limiting factor in achieving high spatial resolution for matrix-assisted laser desorption ionization-mass spectrometry (<a href=";facet-content-type...) imaging is the size of the laser spot at the sample surface. Here, we present modifications to the beam-delivery optics of a commercial <a href=";facet-content-type=Re... ion trap-Orbitrap instrument, incorporating an external Nd:YAG laser, beam-shaping optics, and an aspheric focusing lens, to reduce the minimum laser spot size from ~50&nbsp;μm for the commercial configuration down to ~9&nbsp;μm for the modified configuration. This improved system was applied for <a href=";facet-content-type... imaging of cross sections of juvenile maize leaves at 5-μm spatial resolution using an oversampling method. A variety of different metabolites including amino acids, glycerolipids, and defense-related compounds were imaged at a spatial resolution well below the size of a single cell. Such images provide unprecedented insights into the metabolism associated with the different tissue types of the maize leaf, which is known to asymmetrically distribute the reactions of C4 photosynthesis among the mesophyll and bundle sheath cell types. The metabolite ion images correlate with the optical images that reveal the structures of the different tissues, and previously known and newly revealed asymmetric metabolic features are observed.</p>